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CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level <t>of</t> <t>Angptl4</t> , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by <t>ELISA.</t> I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.
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CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level <t>of</t> <t>Angptl4</t> , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by <t>ELISA.</t> I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.
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CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level <t>of</t> <t>Angptl4</t> , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by <t>ELISA.</t> I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.
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CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level <t>of</t> <t>Angptl4</t> , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by <t>ELISA.</t> I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.
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CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level <t>of</t> <t>Angptl4</t> , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by <t>ELISA.</t> I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.
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CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level <t>of</t> <t>Angptl4</t> , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by <t>ELISA.</t> I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.
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CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

Journal: Materials Today Bio

Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

doi: 10.1016/j.mtbio.2026.103015

Figure Lengend Snippet: CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

Article Snippet: The concentration of Angptl4 was determined via the ELISA method (CUSABIO Co., Ltd., Wuhan, China, cat# CSB-EL001712MO).

Techniques: Cell Function Assay, Tube Formation Assay, Wound Healing Assay, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay